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Literature summary extracted from

  • Keppetipola, N.; Shuman, S.
    Mechanism of the phosphatase component of Clostridium thermocellum polynucleotide kinase-phosphatase (2006), RNA, 12, 73-82.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.78 expressed in Escherichia coli Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Acetivibrio thermocellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 Ni-agarose chromatography Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Acetivibrio thermocellus ADP + 5'-phospho-DNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.78 5' polynucleotide kinase
-
 Acetivibrio thermocellus
2.7.1.78 PNKP
-
 Acetivibrio thermocellus
2.7.1.78 polynucleotide kinase-phosphatase catalyzes ATP-dependent phosphorylation of 5'-OH termini of DNA or RNA polynucleotides and Ni2+/Mn2+-dependent dephosphorylation of 2',3' cyclic phosphate, 2'-phosphate, and 3'-phosphate ribonucleotides Acetivibrio thermocellus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP
-
 Acetivibrio thermocellus